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Methane-oxidizing bacteria (MOB) is a group of planktonic microorganisms ... USA). At least 1 to 3ul of DNA templates were added to the ligation mix depending on the initial cell concentrations after ...

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In addition to the template DNA or cDNA, each reaction included serially diluted plasmids containing the target gene and negative control to ensure that the qPCR assay was stable and uncontaminated.