First, 400 µl of the enzyme was equilibrated with 50 µl of 1 M Tris-HCl buffer (final concentration 50 mM), and diluted with 530 µl sterile water. Next, 20 µl of 4-NPP (final concentration 1 mM) was ...

Department of Chemistry, Gwangju Institute of Science and Technology (GIST), 123 Cheomdan-gwagiro, Buk-gu, Gwangju 61005, Republic of Korea Innovative Energy and Carbon Optimized Synthesis for ...

Department of Materials Science, Graduate School of Engineering, Osaka Metropolitan University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, Japan ...