The researchers tag a fluorescent protein with a known ubiquitin code. Then, they deliver the tagged fluorescent protein to the cells. On the basis of the fluorescence intensity, which correlates ...

Experiments with fluorescence recovery after photobleaching (FRAP) started 30 years ago to visualize the lateral mobility and dynamics of fluorescent proteins in living cells. Its popularity ...

The red fluorescent protein serves as an internal control, whereas the green fluorescent protein is expressed as a fusion with the protein of interest (X). The ratio of red to green fluorescence ...